FastQCFastQC Report
Tue 2 Jul 2024
HFHGFDRX5_n02_YTW257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n02_YTW257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACTACCATGTACAAATAAAAAAAGACTTATTTAAATATACTCATGCTT17.142857142857142No Hit
AAAAAAAAAAAAATGCAAACAAAACAAAAACCTTAAAAAAAATCACCAAA17.142857142857142No Hit
GCTCATACTATACAAAAAATCCTAAAATAAATAATGCTAACCAAAGATCA17.142857142857142No Hit
AAACTTCCTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCAT17.142857142857142No Hit
TTCTGGTCCATATACTTGAAAGGTAAATAATACAAAAAAAGAAAACACAT17.142857142857142No Hit
ATACACTACATCACCTTAATCCATTACCACAATGAATAGTACCAACTTAT17.142857142857142No Hit
AAACTTCCTTCACGATATAAACATAGTCAGATAACAATTGACGCATCAAG17.142857142857142No Hit
ATAAACAAATCTACCAAAACTAAAAATACGCAAAAAATAGATCGATAAAT17.142857142857142No Hit
AGCAGTAGCATGAACGTACCACCCCAATACTATAGACTAAAACAAAAAAA17.142857142857142No Hit
AATAAACATAGAAATAAACTATACCAAAAAATTACCCATTAAATAATTAA17.142857142857142No Hit
GTGCTCTTCCGATTGACGCATGCATTCTTGACATATTGCTTACGACAGAT17.142857142857142No Hit
TCAATTCCGATTGACTAATGAATTCTTTAAATAATCCATACGACAGATCG17.142857142857142No Hit
AAACTTACAAAAACATATAAACAAAGTATAACTAAAATTCAACCAAGCAT17.142857142857142No Hit
TAGACCAAGTTCGACAGAAGTCACATACACAAGCCCCATCTGACTAAAGT17.142857142857142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)