FastQCFastQC Report
Tue 9 Jul 2024
HFHGFDRX5_n01_YTW274_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n01_YTW274_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4534447
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATC366291580.77975109202953No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTAGATCGGAAGAGC1456753.2126298973171368No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACAGATCGGAAG737041.6254242248282975No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTAGA498701.0998033497800284No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATAGATCGGAAGAGCAC389910.8598843475290372No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGAGATCG213130.47002423889837064No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATA177500.39144795385192505No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACACAGACTATC156780.3457532969290412No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACAGAT154750.34127645554132624No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCGGACTATC153620.33878442068018433No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGGCTATC141290.3115925712661323No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTAGATCGGAAGAGCA137570.30338870428963No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGAGATCGGAA130610.2880395338174644No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCATTTTTACGCAGACTATC115770.2553122795348584No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTACC114670.252886404891269No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACCATC95010.20952940898857128No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTGTC92880.20483203354234816No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTCTACGCAGACTATC91580.2019650907817425No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTAGATCGGAAGAG82650.1822713993569668No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTATGCAGACTATC78700.17356030404589579No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTACGCAGACTATCT75090.16559902453375241No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATCTTACGCAGACTATC70440.1553441908131245No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTGCGCAGACTATC67670.1492353973924494No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTCACGCAGACTATC50570.11152407338755972No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCTCAATTTTACGCAGACTATCT48890.1078191012046232No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCATACTATC48730.10746624671101018No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGCAATTTTACGCAGACTATCT48430.1068046445354858No Hit
GTCGTAAGCAATATTTCAAGATGCATGCGTCAATTTTACGCAGACTATCT47630.10504037206742078No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCATCAATTTTACGCAGACTATC47500.10475367779136022No Hit
GTCGTAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATCT47410.1045551971387029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)