FastQCFastQC Report
Sat 31 Aug 2019
HFH77BGXC_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFH77BGXC_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440528019
Sequences flagged as poor quality0
Sequence length92
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGG14498880.32912503574488866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATGCATTGGATGGATGCC10115490.22962194375200456No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8844040.20075998843560502No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGTGCTGGCGACGCATCAT5190880.11783314059757911No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGAT4629060.1050798087828325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT26760200.072.053081
AGCAGTG28584350.067.308822
GCAGTGG34038200.056.47853
AGTGGTA37848400.050.8180435
CAGTGGT37872400.050.7380034
GTGGTAT41367750.046.4763076
GGTATCA42354400.045.373778
TGGTATC42772500.044.922947
GTATCAA43022200.044.6257449
CAACGCA42155350.026.44546912-13
ACGCAGA42455550.026.27950514-15
ATCAACG42678750.026.16665610-11
GCAGAGT42934250.025.98944916-17
AGTACAT43048250.025.79724120-21
AGAGTAC43384350.025.721718-19
TACATGG43133300.025.66585222-23
CATGGGG31169400.024.9777324-25
CGGGCAT2305550.023.48610550-51
TGGGGCC1183750.023.0287626-27
GTACATG42254050.022.68488722-23
Produced by FastQC (version 0.11.5)