FastQCFastQC Report
Sat 31 Aug 2019
HFH77BGXC_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFH77BGXC_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440528019
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTACGC5976950813.567697268309283No Hit
TGACGCCC5780412013.121553569104535No Hit
CCCAGGAT5617688212.752170027123746No Hit
CTGACTGA5178662111.75557938801618No Hit
TAAGTATG4899548911.12199153897632No Hit
ACCGTATG4651031610.557856479952981No Hit
GATTAGAT426548689.682668561429233No Hit
GTTCCTCA413298789.381895411288244ABI Solid3 Adapter A (100% over 8bp)
GGGGGGGG187870004.2646549571685695No Hit
TGCCGCCC8925140.20260096100720443No Hit
CCAGGATA5206300.11818317508653178No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)