FastQCFastQC Report
Sat 31 Aug 2019
HFH77BGXC_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFH77BGXC_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440528019
Sequences flagged as poor quality0
Sequence length29
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA781150.017.9332621
AGCCGAA641200.017.4702578
GGGGGGC496750.017.09167523
TAGCGAC575550.016.5178037
AACGGGA687350.016.4724751
GGGCTAC673950.016.3442331
CTAGCGA667200.016.0484496
GATAGAA1286200.015.9351121
AGACGCC635500.015.8065328
ACGCACG963550.015.7968991
GACGACG513650.015.7502799
CTGTGGG950400.015.6874371
ATGGGAG739800.015.6498341
TGACTCC897550.015.598591
GGCTAGG736700.015.5600894
CGCAGAC524300.015.4874236
AATGATC829150.015.4729033
GTGGGCA647750.015.408443
TCGAGAC552800.015.3338055
AGGATAC876250.015.326038
Produced by FastQC (version 0.11.5)