FastQCFastQC Report
Fri 12 Mar 2021
HFGGKBGXG_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGGKBGXG_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48767507
Sequences flagged as poor quality0
Sequence length49
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28734365.892111729229875No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2763070.5665801206528766Clontech SMART CDS Primer II A (100% over 26bp)
GTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTT2527120.5181974957218953No Hit
CCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGC2291910.4699666111700153No Hit
CGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCT698040.14313628949702104No Hit
CCGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAAT581730.11928639288450812No Hit
GTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGG536620.11003638139632603No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT534950.1096939402705166Clontech SMART CDS Primer II A (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)