FastQCFastQC Report
Fri 12 Mar 2021
HFGGKBGXG_n02_10X_CD8_v2_1_3_ORF_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGGKBGXG_n02_10X_CD8_v2_1_3_ORF_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28834523
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTT12651634.387667519244206No Hit
CCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGC11652324.041100315756914No Hit
CGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCT3564231.236098131396174No Hit
CCGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAAT3029331.0505913345610052No Hit
GTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGG2729660.9466638307143141No Hit
GTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAA1703800.5908889146527584No Hit
CCGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAG1683770.5839423804583138No Hit
CCGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGA1294590.44897222679910465No Hit
GTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGACT1253910.43486413838023263No Hit
CGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATG1113340.3861135486791302No Hit
GTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCA1054700.36577681552075614No Hit
ACCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGG1026560.3560176806115364No Hit
CCGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCAC1006840.3491786564320832No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT856200.29693572527626Clontech SMART CDS Primer II A (100% over 26bp)
GTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTCCT780860.27080732356834897No Hit
TGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTG778580.27001660474841216No Hit
CCGTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTC724150.2511399269549214No Hit
GGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGTT695230.2411102829757232No Hit
CGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGA559980.19420470385447333No Hit
CCGTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAG464120.16095983276713127No Hit
GTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGCA457170.15854952759232396No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG452190.15682243122246206No Hit
CGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGAC397480.137848647608979No Hit
GTGGGCTTGTACTCTTCCAACGACAGGCCACTGAAGCTGGTGTCTCTGG396590.13753998982400367No Hit
GTGGGCTTGTACTCACTGGAATGGCATTTTTTTTGGCATTGACAACCTG370660.12854729727972264No Hit
CCGTGGGCTTGTACTCACTGGAATGGCATTTTTTTTGGCATTGACAACC344280.11939854181045409No Hit
CCGTGGGCTTGTACTCACGCATGATGTGATCTGTTTAAATACTGACGAT343500.11912803274047573No Hit
GGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGT343090.11898584207548708No Hit
CCGTGGGCTTGTACTCTTCCAACGACAGGCCACTGAAGCTGGTGTCTCT337920.11719285247063042No Hit
GTGGGCTTGTACTCCTCGTTCATGGTGATCACCCCGCGCACGTTCTCGT329780.11436984756085612No Hit
CCGTGGGCTTGTACTCCATTCCCCACTTAAGTTTCACCATCACTGGCTT323190.11208439272603886No Hit
CGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACC319890.11093993127613035No Hit
CCGTGGGCTTGTACTCCTCGTTCATGGTGATCACCCCGCGCACGTTCTC314970.10923364329626677No Hit
GTGGGCTTGTACTCCATTCCCCACTTAAGTTTCACCATCACTGGCTTTC292600.10147558189188702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)