FastQCFastQC Report
Fri 12 Mar 2021
HFGGKBGXG_n02_10X_CD8_v2_1_3_ORF_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGGKBGXG_n02_10X_CD8_v2_1_3_ORF_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26283892
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTT11524744.3847159317197No Hit
CCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGC10641294.0485975212498975No Hit
CGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCT3251031.2368906400924184No Hit
CCGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAAT2740251.0425586895578478No Hit
GTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGG2480900.9438860881029338No Hit
GTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAA1548850.5892772653304161No Hit
CCGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAG1525500.5803934972796266No Hit
CCGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGA1181770.4496175832711533No Hit
GTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGACT1142980.4348594949332466No Hit
CGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATG1006660.3829950298076099No Hit
GTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCA959200.3649383432255771No Hit
ACCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGG939150.35731009699781147No Hit
CCGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCAC919560.34985686290295215No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT795810.3027747945395606Clontech SMART CDS Primer II A (100% over 26bp)
TGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTG711220.27059158514271786No Hit
GTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTCCT707870.26931704026176945No Hit
CCGTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTC656190.24965480759090017No Hit
GGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGTT631580.24029165848041073No Hit
CGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGA507700.19316013016641523No Hit
CCGTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAG420060.15981651423617171No Hit
GTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGCA417760.15894145357164No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG401820.15287690270527668No Hit
GTGGGCTTGTACTCTTCCAACGACAGGCCACTGAAGCTGGTGTCTCTGG361010.137350282827216No Hit
CGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGAC359000.136585555898647No Hit
GTGGGCTTGTACTCACTGGAATGGCATTTTTTTTGGCATTGACAACCTG336100.12787299536917895No Hit
CCGTGGGCTTGTACTCACGCATGATGTGATCTGTTTAAATACTGACGAT311990.11870007683793557No Hit
CCGTGGGCTTGTACTCACTGGAATGGCATTTTTTTTGGCATTGACAACC311880.11865822611050143No Hit
GGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGT311250.11841853558065146No Hit
CCGTGGGCTTGTACTCTTCCAACGACAGGCCACTGAAGCTGGTGTCTCT310400.11809514359593319No Hit
GTGGGCTTGTACTCCTCGTTCATGGTGATCACCCCGCGCACGTTCTCGT301510.11471284389693885No Hit
CCGTGGGCTTGTACTCCATTCCCCACTTAAGTTTCACCATCACTGGCTT294800.11215994952345718No Hit
CGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACC292690.11135717647903895No Hit
CCGTGGGCTTGTACTCCTCGTTCATGGTGATCACCCCGCGCACGTTCTC286390.10896027118053901No Hit
GTGGGCTTGTACTCCATTCCCCACTTAAGTTTCACCATCACTGGCTTTC267870.1019141305252662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)