FastQCFastQC Report
Fri 12 Mar 2021
HFGGKBGXG_n02_10X_CD8_v2_1_1_ORF_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGGKBGXG_n02_10X_CD8_v2_1_1_ORF_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19727299
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTT10293755.218023004568441No Hit
CCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGC9157494.642039439864525No Hit
CGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCT2774631.4064925968831312No Hit
CCGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAAT2294771.163245916230093No Hit
GTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGG2151251.090493939388256No Hit
GTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAA1625710.8240915292052906No Hit
CCGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAG1534240.7777243098510344No Hit
GTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCA1189520.6029816854299213No Hit
CCGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCAC1093580.5543485704758669No Hit
GTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGACT913420.4630233464804279No Hit
CCGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGA898730.4555768126188993No Hit
CGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATG848420.4300740816064075No Hit
ACCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGG757830.3841529446073687No Hit
TGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTG675930.3426368708661029No Hit
GGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGTT580920.2944751838556307No Hit
CGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGA510240.2586466601433881No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT508190.25760749102043823Clontech SMART CDS Primer II A (100% over 26bp)
GTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTCCT401010.20327668780201485No Hit
CCGTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTC358320.18163662445629278No Hit
CGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACC346510.1756499964845669No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344530.1746463111853275No Hit
GTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGCA307010.15562698167650826No Hit
CCGTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAG301910.1530417316633159No Hit
GGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGT283940.14393252720506744No Hit
CCGTGGGCTTGTACTCACGCATGATGTGATCTGTTTAAATACTGACGAT283120.1435168595558875No Hit
CGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGAC275420.1396136389477343No Hit
CCGTGGGCTTGTACTCCATTCCCCACTTAAGTTTCACCATCACTGGCTT253770.128638999185849No Hit
GTGGGCTTGTACTCACTGGAATGGCATTTTTTTTGGCATTGACAACCTG250860.1271638859430275No Hit
GTGGGCTTGTACTCTTCCAACGACAGGCCACTGAAGCTGGTGTCTCTGG247860.1256431506411496No Hit
GTGGGCTTGTACTCCATTCCCCACTTAAGTTTCACCATCACTGGCTTTC238460.12087818002859896No Hit
GCCGTGGGCAAGACCTGCCTTCTCATCAGCTACACCACCAACGCCTTTC237450.1203661991436334No Hit
GTGGGCTTGTACTCCTCGTTCATGGTGATCACCCCGCGCACGTTCTCGT235330.11929154619697303No Hit
CCGTGGGCTTGTACTCACTGGAATGGCATTTTTTTTGGCATTGACAACC222420.11274731528122528No Hit
CCGTGGGCTTGTACTCCTCGTTCATGGTGATCACCCCGCGCACGTTCTC216510.10975146673652587No Hit
CCGTGGGCTTGTACTCTTCCAACGACAGGCCACTGAAGCTGGTGTCTCT204680.10375470052945412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)