FastQCFastQC Report
Fri 12 Mar 2021
HFGGKBGXG_n02_10X_CD8_v2_1_1_ORF_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGGKBGXG_n02_10X_CD8_v2_1_1_ORF_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21128312
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTT10970045.192104319549995No Hit
CCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGC9772954.625523326236379No Hit
CGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCT2969281.4053559981507278No Hit
CCGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAAT2456101.1624686345033148No Hit
GTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGG2311341.093953932524283No Hit
GTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAA1745100.8259533463913254No Hit
CCGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAG1650170.7810231124947417No Hit
GTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCA1274350.6031480413579655No Hit
CCGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCAC1174510.555893911449244No Hit
GTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGACT978150.4629570029068105No Hit
CCGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGA970130.45916114832079347No Hit
CGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATG910290.43083896148447637No Hit
ACCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGG811600.38412912493908646No Hit
TGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTG715860.3388155191952864No Hit
GGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGTT617480.29225240520870766No Hit
CGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGA552600.2615447935452676No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT548470.25959007042304183Clontech SMART CDS Primer II A (100% over 26bp)
GTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTCCT431420.20419047200741827No Hit
CCGTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTC384690.18207322951308177No Hit
CGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACC373610.17682908128202576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG366650.17353492318742736No Hit
GTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGCA331370.15683694939756665No Hit
CCGTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAG320680.15177738761146656No Hit
GGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGT305350.14452172042896755No Hit
CCGTGGGCTTGTACTCACGCATGATGTGATCTGTTTAAATACTGACGAT303190.1434993955030577No Hit
CGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGAC290940.13770148793713383No Hit
CCGTGGGCTTGTACTCCATTCCCCACTTAAGTTTCACCATCACTGGCTT270380.12797046919791794No Hit
GTGGGCTTGTACTCTTCCAACGACAGGCCACTGAAGCTGGTGTCTCTGG266520.1261435366914309No Hit
GTGGGCTTGTACTCACTGGAATGGCATTTTTTTTGGCATTGACAACCTG263390.12466211214601525No Hit
GCCGTGGGCAAGACCTGCCTTCTCATCAGCTACACCACCAACGCCTTTC253130.11980606874794353No Hit
GTGGGCTTGTACTCCATTCCCCACTTAAGTTTCACCATCACTGGCTTTC252960.1197256079898858No Hit
GTGGGCTTGTACTCCTCGTTCATGGTGATCACCCCGCGCACGTTCTCGT251530.11904879102504734No Hit
CCGTGGGCTTGTACTCACTGGAATGGCATTTTTTTTGGCATTGACAACC239380.11329821331680448No Hit
CCGTGGGCTTGTACTCCTCGTTCATGGTGATCACCCCGCGCACGTTCTC234020.11076133294510228No Hit
CCGTGGGCTTGTACTCTTCCAACGACAGGCCACTGAAGCTGGTGTCTCT215460.10197691135950661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)