FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38845851
Sequences flagged as poor quality0
Sequence length47
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18175994.679004200474331No Hit
ATTGACCCGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2801540.7211941373095417No Hit
ATTGACCCGCGTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1804490.46452579967935315No Hit
ATTGACCCGCGTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1457310.37515203361100263No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1391890.35831111023928913No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1202490.3095542944856582No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1184260.30486138661243384No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160360.29870886339959446No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1103930.28418221549580674No Hit
TGCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA981330.25262157340818714No Hit
CAGTAGTCACGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA798560.20557150363368276No Hit
TGCAATTACCCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA792870.20410673973907792No Hit
CTCCTCTGCAATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA597020.15368951500122882No Hit
TGCAATTACCCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550780.14178605586475632No Hit
ATTGACCCGCGTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524730.1350800629905109No Hit
CTCCTCTGCAATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA503340.12957368342889436No Hit
ACTTCCGTCGATCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA401610.10338555847315585No Hit
GAGGTTAGTGATGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390200.10044830785146142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG1005500.040.7390025
GTCACGG797850.040.6566626
GCGTTAG990600.040.6185849
CGCGTTA1018650.040.6017158
GACCCGC1040800.040.537974
AGTAGTC817800.040.532252
CCCGCGT988500.040.5115286
TCACGGT745900.040.4988447
GTAGTCA812350.040.492443
CACGGTC737750.040.406768
CGTTAGC287200.040.21941410
TGACCCG1056850.040.0990833
CGACGTA99250.039.9454844
TACCCGG332000.039.8964967
TCCGTCG136100.039.808964
ACCCGGA333200.039.804398
CCCGGAT323800.039.8025559
GACGTAG97450.039.590535
GTTAGCA293400.039.42061611
CGGTCAT194200.039.2838310