FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_c-PBMCpilot-rna-lic-1.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_c-PBMCpilot-rna-lic-1.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20843041
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238830.11458500705343333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT918100.037.455141
AGCAGTG991400.034.726992
GTGGTAT1152200.029.8670526
CAGTGGT1161550.029.6719364
TCAACGC1148050.029.51854112
ATCAACG1149750.029.476211
AGTGGTA1171800.029.4326935
CAACGCA1152700.029.38352613
TGGTATC1175000.029.1870087
GGTATCA1184900.028.8203748
CGCAGAG1184850.028.67794816
GTATCAA1186600.028.5897739
GCAGTGG1209500.028.5625783
TATCAAC1190250.028.52346810
GAGTACA1178950.028.3982120
GTACATG1183750.028.32734922
GCAGAGT1195850.028.30222517
TACATGG1185850.028.1486923
AGTACAT1187900.028.02849221
CAGAGTA1212800.027.85201818
Produced by FastQC (version 0.11.5)