FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_c-PBMCpilot-rna-lib2.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_c-PBMCpilot-rna-lib2.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19815505
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG224190.11313867600144431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT754600.036.6949651
AGCAGTG810100.034.1792142
GTGGTAT916300.030.224076
TCAACGC905250.030.00935412
ATCAACG909250.029.89604811
CAACGCA909300.029.88944813
CAGTGGT934100.029.6940124
TGGTATC934300.029.5432667
AGTGGTA941400.029.4955025
GGTATCA943150.029.1941368
CGCAGAG937200.029.13048416
GTATCAA946550.028.8305179
TATCAAC948550.028.71748710
GAGTACA940500.028.66346720
GTACATG940450.028.59522422
GCAGAGT955000.028.54815917
TACATGG937900.028.4454723
AGTACAT949250.028.24121921
GCAGTGG987650.028.1908023
CAGAGTA973450.027.97422818
Produced by FastQC (version 0.11.5)