FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_c-PBMCpilot-rna-lib2.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_c-PBMCpilot-rna-lib2.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21514571
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240630.11184513044670981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT823800.036.643241
AGCAGTG881000.034.2625922
GTGGTAT991500.030.5282156
TCAACGC981350.030.27165412
ATCAACG983500.030.20962311
CAACGCA987300.030.10798313
CAGTGGT1012750.029.9222134
TGGTATC1013300.029.7756397
AGTGGTA1021100.029.7128985
GGTATCA1020200.029.4510368
CGCAGAG1015750.029.31731216
GTATCAA1023000.029.1346869
GTACATG1008200.029.09064322
GAGTACA1006900.029.05095720
TATCAAC1027100.029.02948410
GCAGAGT1025850.028.86816217
TACATGG1014200.028.79657223
AGTACAT1018600.028.64661421
GCAGTGG1072000.028.317523
CAGAGTA1048200.028.19303718
Produced by FastQC (version 0.11.5)