FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_c-PBMCpilot-rna-lib2.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_c-PBMCpilot-rna-lib2.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25203469
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280110.1111394625874716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT959200.036.693711
AGCAGTG1027450.034.2212942
GTGGTAT1163550.030.2919986
TCAACGC1148350.030.04072212
ATCAACG1153350.029.93305611
CAACGCA1153150.029.92652313
CAGTGGT1187200.029.6686344
TGGTATC1188000.029.5623427
AGTGGTA1198550.029.449245
GGTATCA1196600.029.2467618
CGCAGAG1189950.029.17667416
GTATCAA1203200.028.8417079
GAGTACA1188450.028.81943320
TATCAAC1206550.028.72894510
GTACATG1189400.028.70803822
GCAGAGT1209000.028.64870317
TACATGG1190850.028.5072823
AGTACAT1202000.028.37304521
GCAGTGG1254800.028.1639823
CAGAGTA1237250.027.97910718
Produced by FastQC (version 0.11.5)