FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_c-PBMCpilot-rna-lib2.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_c-PBMCpilot-rna-lib2.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20148510
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220120.10924877323434834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT768350.036.674911
AGCAGTG817850.034.43772
GTGGTAT928150.030.3377276
TCAACGC917750.030.14728412
CAACGCA921150.030.05841813
ATCAACG922300.030.05723611
CAGTGGT942250.029.9425854
TGGTATC945800.029.6767967
AGTGGTA952850.029.619275
CGCAGAG945100.029.27941716
GGTATCA957300.029.2339388
GTATCAA957650.029.0482069
TATCAAC959900.028.9455110
GAGTACA949800.028.87519520
GTACATG949650.028.83203922
GCAGAGT964200.028.71196217
TACATGG952750.028.60014223
GCAGTGG991850.028.4998263
AGTACAT962350.028.41532721
CAGAGTA985500.028.07552118
Produced by FastQC (version 0.11.5)