FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_c-PBMCpilot-rna-lib1.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_c-PBMCpilot-rna-lib1.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20358161
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG251630.12360153748661286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT886900.037.2422681
AGCAGTG958150.034.492512
GTGGTAT1114900.029.7606536
CAGTGGT1121350.029.5967164
TCAACGC1094750.029.4226312
ATCAACG1098000.029.35750611
AGTGGTA1133950.029.310155
CAACGCA1101450.029.24773813
TGGTATC1131300.029.178677
GGTATCA1140250.028.8126968
GCAGTGG1161650.028.6499923
GTATCAA1133850.028.5638479
CGCAGAG1140100.028.54585516
TATCAAC1137450.028.4190210
GAGTACA1129100.028.32538620
GTACATG1135400.028.31151222
GCAGAGT1150150.028.21584717
TACATGG1139900.028.09133323
AGTACAT1138650.027.9867721
CAGAGTA1166250.027.68683418
Produced by FastQC (version 0.11.5)