FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_c-PBMCpilot-rna-lib1.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_c-PBMCpilot-rna-lib1.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21411031
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245940.11486602396680477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT963900.037.256141
AGCAGTG1036550.034.6654742
GTGGTAT1200050.029.9462536
CAGTGGT1211350.029.7315144
TCAACGC1190550.029.59290712
AGTGGTA1219450.029.5094325
ATCAACG1193950.029.50903111
CAACGCA1195600.029.4697613
TGGTATC1221900.029.2770847
GGTATCA1233750.028.9192038
CGCAGAG1230850.028.78064716
GTATCAA1231850.028.6871459
GCAGTGG1259150.028.668253
TATCAAC1236300.028.6047810
GAGTACA1227100.028.47143720
GCAGAGT1243650.028.38345517
GTACATG1231650.028.35920122
TACATGG1233100.028.17893223
AGTACAT1238200.028.08995221
CAGAGTA1265250.027.88542618
Produced by FastQC (version 0.11.5)