FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_c-PBMCpilot-rna-lib1.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_c-PBMCpilot-rna-lib1.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22469551
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258750.11515583911756848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT996200.037.261711
AGCAGTG1073000.034.5705832
GTGGTAT1243450.029.8492986
CAGTGGT1256800.029.5872124
TCAACGC1235400.029.51671412
ATCAACG1239450.029.43338411
AGTGGTA1269100.029.3272155
CAACGCA1244850.029.3109613
TGGTATC1266950.029.2218557
GGTATCA1278100.028.891618
GTATCAA1272600.028.7115929
CGCAGAG1282650.028.59851516
TATCAAC1280800.028.55287710
GCAGTGG1306150.028.5447623
GAGTACA1274500.028.29175220
GCAGAGT1291900.028.23929617
GTACATG1280100.028.20937522
TACATGG1281050.028.01842723
AGTACAT1285950.027.96962521
CAGAGTA1308350.027.79126518
Produced by FastQC (version 0.11.5)