FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_c-PBMCpilot-HTO2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_c-PBMCpilot-HTO2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40371593
Sequences flagged as poor quality0
Sequence length47
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG542358113.434151582772568No Hit
ATTGACCCGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA413584710.244448367444901No Hit
ATTGACCCGCGTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25912876.418589922869776No Hit
ATTGACCCGCGTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21646895.3619112825198645No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17404584.311095675615277No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16398194.0618139591370594No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15550913.851943617880028No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15315303.793583275250001No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12121903.0025815429180613No Hit
CTCCTCTGCAATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8847102.191417118467433No Hit
CAGTAGTCACGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8619722.135095337952109No Hit
ATTGACCCGCGTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7209591.7858076593608778No Hit
CTCCTCTGCAATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7100901.7588852637051007No Hit
CAGTAGTCACGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2204260.545992822230225No Hit
CTCCTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1963520.48636178413866404No Hit
ATTGACCCGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1279750.3169926933524768No Hit
ATTGACCCGCGTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1118500.27705124244168416No Hit
ATTGACCCGCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1107820.2744058179720577No Hit
ATTGACCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1013590.2510651487049322No Hit
ATTGACCCGCGTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT949220.23512076920026415No Hit
ATTGACCCGCGTTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA745930.18476605567682206No Hit
ATTGACCCGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC703310.17420912769035396No Hit
ATTGACCCGCGTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC686410.1700230159359825No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT646260.16007790428284563No Hit
ATTGACACGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA614080.15210695302511348No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT574750.14236495448668574No Hit
ATTGACCCGCGTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT568860.14090600784566515No Hit
ATTGACCCGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT568450.14080445128831057No Hit
ATTGACCCGCGTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC567330.14052702849748833No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT544440.1348572002100586No Hit
ATTGACCCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA503690.1247634692046955No Hit
CAGTAGTCACGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT489170.12116688088082132No Hit
ATTGACCCGCGTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT475430.11776349771484122No Hit
CTCCTCTGCAATTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450360.1115536857809896No Hit
CTCCTCTGCAATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT439450.10885129055967645No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT415760.10298330313594513No Hit
ATTGACCCGCGTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT415610.10294614829788856No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC408280.10113051521152508No Hit
ATTGACCCGCGTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG404330.10015210447603591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAC506.002665E-1141.0063069
CAGTAGT8547700.040.975681
ATTGACC14976500.040.96671
CTCCTCT8201350.040.9666251
AGTAGTC8543050.040.9611852
TCCTCTG8133000.040.9608732
TTGACCC14835750.040.952312
TGACCCG14659050.040.940793
TGACACG218950.040.9402663
GACCCGC14602650.040.9261444
GTAGTCA8549250.040.9225963
TAGTCAC8566350.040.9219254
AGTCACG8523100.040.8948675
ACCCGCG14173950.040.8929025
CCTCTGC8051850.040.8826453
GTCACGG8428450.040.8765956
CTCTGCA8136950.040.8599624
CCCGCGT14017750.040.859816
TCACGGT7890100.040.856927
TCACGGC470950.040.8549427
Produced by FastQC (version 0.11.5)