FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_c-PBMCpilot-HTO1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_c-PBMCpilot-HTO1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42682995
Sequences flagged as poor quality0
Sequence length47
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG562141413.170148908247887No Hit
ATTGACCCGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA435772610.209513179663237No Hit
ATTGACCCGCGTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27936796.545180346411961No Hit
ATTGACCCGCGTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22832815.349392656255729No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17816004.174027619195888No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17089044.003711548357841No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16508683.8677417083782433No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15807103.7033717994718973No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12476582.9230797885668522No Hit
CAGTAGTCACGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9455372.215254576207691No Hit
CTCCTCTGCAATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9172132.1488955964781757No Hit
ATTGACCCGCGTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8122391.9029569035631169No Hit
CTCCTCTGCAATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7807861.8292671355419177No Hit
CAGTAGTCACGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2473440.5794907316133744No Hit
CTCCTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2264930.5306398953494244No Hit
ATTGACCCGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1348770.31599703816473046No Hit
ATTGACCCGCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1328470.3112410457607298No Hit
ATTGACCCGCGTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1197670.28059652327583856No Hit
ATTGACCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1151270.2697256834952655No Hit
ATTGACCCGCGTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT967900.22676478068139314No Hit
ATTGACCCGCGTTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA782170.18325096446488817No Hit
ATTGACCCGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC729200.1708408699998676No Hit
ATTGACCCGCGTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC729130.17082447002606074No Hit
ATTGACACGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA686020.16072442901441195No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT662320.15517186645407616No Hit
ATTGACCCGCGTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT614820.1440433127993947No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT610640.14306400007778275No Hit
ATTGACCCGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT605830.14193708759190868No Hit
ATTGACCCGCGTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC591580.13859852149550425No Hit
ATTGACCCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA571580.1339128146935331No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT548060.12840242349441505No Hit
CAGTAGTCACGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT535880.12554882805201464No Hit
ATTGACCCGCGTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT484840.1135909042933843No Hit
CTCCTCTGCAATTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA469780.11006256707150003No Hit
CAGTAGTCACGGTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA469250.10993839584124779No Hit
CTCCTCTGCAATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT465120.10897079738664076No Hit
ATTGACCCGCGTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT453760.10630931592312114No Hit
ATTGACCCGCGTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG436880.1023545793822575No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT434870.1018836658486594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTCT8539400.040.9726941
CAGTAGT9076350.040.971221
ATTGACC15970000.040.9641951
TCCTCTG8465900.040.9565962
TTGACCC15812200.040.9497572
AGTAGTC9088600.040.945982
TGACCCG15624500.040.9401863
GACCCGC15557150.040.9282884
GTAGTCA9093000.040.9194453
TAGTCAC9104150.040.9148524
ACCCGCG15109100.040.8958785
CCTCTGC8364750.040.8852463
AGTCACG9064800.040.8786355
GTCACGG8965500.040.86646
CTCTGCA8474750.040.8663184
CCCGCGT14929150.040.8656
TCACGGC537800.040.8649567
TCTGCAA8554600.040.8577775
TTGACAC258550.040.849972
TCACGGT8360700.040.8479547
Produced by FastQC (version 0.11.5)