FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_c-PBMCpilot-BulkA1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_c-PBMCpilot-BulkA1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17338457
Sequences flagged as poor quality0
Sequence length47
%GC18

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCACTGCAATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13762797.937724792927075No Hit
CAGCCCACTGCAATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6814843.930476627764512No Hit
CAGCCCACTGCAATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5854653.376684557339791No Hit
TGCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3676092.120194432526493No Hit
TGCAATTACCCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2849331.643358460328967No Hit
TGCAATTACCCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2646871.5265891307398347No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1702720.9820481718759634No Hit
GAGGTTAGTGATGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1263910.7289633673861521No Hit
TGGTAACGACAGTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1213560.699923874425504No Hit
AATCCTTCCGAATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1212520.6993240517307855No Hit
AATCCTTCCGAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1157650.6676776370584764No Hit
GAGGTTAGTGATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1131610.6526589995868721No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1087300.6271030922763197No Hit
GAGGTTAGTGATGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1075120.6200782457170209No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA999440.576429609624432No Hit
AATCCTTCCGAATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA979580.5649753031656738No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA956280.5515369677936162No Hit
TGGTAACGACAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA947110.5462481465334545No Hit
TGGTAACGACAGTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA945190.5451407815585897No Hit
ACTTCCGTCGATCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA906820.5230107846390253No Hit
GCTGCGCTTTCCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750840.43304891548307906No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG690200.39807463836026474No Hit
CTCATTGTAACTCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA688680.39719797442183No Hit
CTCATTGTAACTCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA679510.39190915316166836No Hit
GTCTTTGTCAGTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA664310.3831425137773217No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA644400.3716593696890098No Hit
GGGTTGTTGTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA634520.36596105408918456No Hit
ACTTCCGTCGATCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA609820.3517152650896213No Hit
TATCCCTTGGGATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA603040.3478048825221298No Hit
GCTGCGCTTTCCATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA596270.34390026748054914No Hit
CTCATTGTAACTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560150.3230679638909045No Hit
GGGTTGTTGTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA543750.3136092213972673No Hit
CTGGGCAATTACTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA519570.29966334374506337No Hit
ACCTTTATGCCACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA517530.29848676845926947No Hit
GGGTTGTTGTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA514530.29675651068604314No Hit
GCTGCGCTTTCCATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA511100.2947782492986544No Hit
ACCTTTATGCCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA501550.28927026205388406No Hit
TATCCCTTGGGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA488180.2815590799112055No Hit
ACCTTTATGCCACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA484040.2791713241841532No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA463660.26741710637803584No Hit
GTCTTTGTCAGTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA422150.24347610632249453No Hit
ACTTCCGTCGATCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA416620.24028666449384742No Hit
CTGGGCAATTACTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA416130.24000405572422046No Hit
CAGCCCACTGCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA415400.23958302633273537No Hit
GTCTTTGTCAGTGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA405200.23370014990376592No Hit
TGACCTTCCTCTCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA398510.22984167506947129No Hit
TATCCCTTGGGATGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA398350.2297493946548992No Hit
CAGCCCACTGCAATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT388000.22378000533726847No Hit
CTGGGCAATTACTCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA377250.21757991498320756No Hit
GATTAACCAGCTCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA361570.20853643435514474No Hit
TGACCTTCCTCTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA351700.2028438862812302No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA346730.19997742590358533No Hit
CAGCCCACTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA329440.19000537360389105No Hit
TGACCTTCCTCTCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA327070.18863846996304226No Hit
CTGATAGACTTGAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA326730.18844237408207662No Hit
GATTAACCAGCTCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA325410.18768106066185705No Hit
AACTCCCTATGGAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA322310.1858931276295232No Hit
CAGCCCACTGCAATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC311140.1794508011872106No Hit
CAGCCCACTGCAATAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA264810.15272985364268574No Hit
AACTCCCTATGGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA263100.15174360671194673No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252850.14583189265342356No Hit
CAGTTCCTCAGTTCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA251830.1452436050105266No Hit
TGAAGGCTCATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA251660.14514555707004378No Hit
TACGATTTGTCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA249790.14406702972473273No Hit
CAGTTCCTCAGTTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248530.14334032145997766No Hit
GATTAACCAGCTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA241290.13916463270059154No Hit
CAGCCCACTGCAATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT240480.13869746310182043No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239600.13818992082167403No Hit
CTATCGTTTGATGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA237660.1370710207949877No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA237080.13673650429216394No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA237040.13671343418852092No Hit
TGCAATTACCCGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA236740.1365404084111983No Hit
GCGCTACTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA236340.13630970737476814No Hit
CAGCCCACTGCAATACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA235240.13567527952458516No Hit
CAGCCCACTGCAATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC229800.13253774542913477No Hit
TCACCAGTACCTAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA226400.1305767866194783No Hit
GCGCTACTTCCTTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA221790.12791795717462057No Hit
CAGTTCCTCAGTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220920.12741618242038494No Hit
CAGCCCACTGCAATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT220180.1269893855029891No Hit
AACTCCCTATGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA217010.12516107978927998No Hit
ATTTCCATTCCCTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA215880.12450934936136474No Hit
GTATCCTTCCTTGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212390.12249648281851147No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210920.12164865650963058No Hit
CAGCCCACTGCAATATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA208790.12042017349063992No Hit
TCAGGTCCTTTCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA204950.11820544354091025No Hit
TATTGGGACACTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA204490.11794013734901555No Hit
CTGATAGACTTGAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA203150.11716728887697446No Hit
CAGCCCACTGCAATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA203130.11715575382515295No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200890.11586382802114398No Hit
TCAGGTCCTTTCAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199840.11525823780051477No Hit
GCGCTACTTCCTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199020.11478530067583292No Hit
CCTTCCGTATCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193690.11171120936540085No Hit
CAGCCCACTGCAATAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA183760.10598405613602178No Hit
CCAAAGTAAGAGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA183330.10573605252185934No Hit
TATTGGGACACTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182290.1051362298271409No Hit
CGTGTTTAACTTGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182240.10510739219758714No Hit
CAGCCCACTGCAATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181350.10459408239153No Hit
CAGCCCACTGCAATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC180740.10424226331097398No Hit
CTGATAGACTTGAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180550.10413268031866967No Hit
TGGCTTCAGGTCCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180050.10384430402313194No Hit
TCAGGTCCTTTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA179580.10357323030532647No Hit
TGTACCCGCTTGTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA178110.10272540399644559No Hit
TACGATTTGTCAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177310.10226400192358526No Hit
CTATCGTTTGATGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175780.10138157045923983No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174900.10087402817909345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT256.7323024E-541.01082213
CTCGTAC1100.041.01070412
GGGTCTA459.586074E-1041.01070412
GGGTCAT200.001111175241.01070412
GTAGGAC200.001111175241.01070412
TTCTTAC256.7324174E-541.01070412
CAGGCAT256.7324174E-541.01070412
CTCGCAG3150.041.01070412
GTGAGAC200.001111175241.01070412
AGCCTAG256.7324174E-541.01070412
TAAGCTA401.5494152E-841.01070412
GTATTAG304.1039584E-641.010712
GGGTCGA352.5156078E-741.010712
TTGCTAG304.1039584E-641.010712
CCTACAG650.041.010712
GATATGA304.1039584E-641.010712
TAGGGCA352.5156078E-741.010712
TGTAGAG304.1039584E-641.010712
CGTCCAG304.1039584E-641.010712
CGCCCGA650.041.010712
Produced by FastQC (version 0.11.5)