FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-rna-lib2.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-rna-lib2.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19815505
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG224190.11313867600144431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT758600.036.6719471
AGCAGTG812650.034.2258032
GTGGTAT922050.030.1846246
TCAACGC909250.030.03736312
ATCAACG913350.029.91889411
CAACGCA913450.029.91294513
CAGTGGT937100.029.7368284
TGGTATC939150.029.545757
AGTGGTA946150.029.4905645
GGTATCA947300.029.2090728
CGCAGAG942050.029.12843116
GTATCAA950400.028.8496289
TATCAAC952250.028.75216310
GAGTACA943600.028.70176320
GTACATG944100.028.60845422
GCAGAGT959500.028.54874217
TACATGG941250.028.46403123
AGTACAT953000.028.27200521
GCAGTGG991100.028.2126883
AACGCAG982100.027.99736814
Produced by FastQC (version 0.11.5)