FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-rna-lib2.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-rna-lib2.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21514571
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240630.11184513044670981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT824800.036.6387371
AGCAGTG883100.034.2065542
GTGGTAT995500.030.4408326
TCAACGC984500.030.19116612
ATCAACG986700.030.12798311
CAACGCA990800.030.01582313
CAGTGGT1017750.029.813614
TGGTATC1016550.029.7108967
AGTGGTA1024850.029.6225155
GGTATCA1024750.029.350418
CGCAGAG1020900.029.21727416
GTATCAA1027600.029.0243439
GTACATG1012900.029.00767522
GAGTACA1011650.028.98690220
TATCAAC1030400.028.94227210
GCAGAGT1031300.028.7748417
TACATGG1018400.028.7195523
AGTACAT1023600.028.58820321
GCAGTGG1076350.028.2432613
CAGAGTA1052650.028.13552318
Produced by FastQC (version 0.11.5)