FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-rna-lib2.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-rna-lib2.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25203469
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280110.1111394625874716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT956900.036.7260361
AGCAGTG1025950.034.2412572
GTGGTAT1162200.030.3045226
TCAACGC1149550.030.0181312
ATCAACG1154500.029.908311
CAACGCA1154450.029.90326913
CAGTGGT1185350.029.697974
TGGTATC1186900.029.5848757
AGTGGTA1198350.029.4391355
GGTATCA1196750.029.2484918
CGCAGAG1190300.029.16794616
GTATCAA1203800.028.8395089
GAGTACA1189000.028.79905920
TATCAAC1206200.028.73050710
GTACATG1189450.028.69289622
GCAGAGT1208450.028.65305717
TACATGG1192100.028.49100523
AGTACAT1202550.028.35321821
GCAGTGG1254700.028.1632083
CAGAGTA1235900.027.99282618
Produced by FastQC (version 0.11.5)