FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-rna-lib2.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-rna-lib2.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20148510
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220120.10924877323434834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT770150.036.64521
AGCAGTG818950.034.4416962
GTGGTAT928750.030.3642756
TCAACGC919000.030.18007512
CAACGCA922150.030.09714513
ATCAACG924100.030.06519911
CAGTGGT943450.029.963414
TGGTATC948050.029.679547
AGTGGTA953050.029.6582495
CGCAGAG946050.029.34771216
GGTATCA959550.029.2419668
GTATCAA960050.029.0437349
TATCAAC961650.028.95425410
GAGTACA951750.028.91292820
GTACATG951500.028.87304322
GCAGAGT965400.028.76776317
TACATGG954350.028.63177723
AGTACAT962650.028.50013521
GCAGTGG995300.028.461073
CAGAGTA987200.028.11034618
Produced by FastQC (version 0.11.5)