FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-rna-lib1.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-rna-lib1.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20358161
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG251630.12360153748661286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT880750.037.225291
AGCAGTG952350.034.4487652
GTGGTAT1109100.029.7184146
CAGTGGT1115500.029.5423454
TCAACGC1088600.029.39700112
ATCAACG1091950.029.33267211
AGTGGTA1128900.029.248765
CAACGCA1094900.029.23195813
TGGTATC1124450.029.159587
GGTATCA1133850.028.7673078
GCAGTGG1155500.028.5984553
GTATCAA1127900.028.5146359
CGCAGAG1133800.028.50941716
TATCAAC1129550.028.43095410
GAGTACA1122700.028.29711320
GTACATG1129750.028.2699122
GCAGAGT1143250.028.18194217
TACATGG1134000.028.05311423
AGTACAT1133900.027.92344321
CAGAGTA1159950.027.64641218
Produced by FastQC (version 0.11.5)