FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-rna-lib1.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-rna-lib1.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21411031
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245940.11486602396680477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT962050.037.261821
AGCAGTG1036150.034.6196672
GTGGTAT1197450.029.944716
CAGTGGT1207750.029.7488424
TCAACGC1188300.029.60730712
ATCAACG1192150.029.51072111
AGTGGTA1216750.029.5042865
CAACGCA1193100.029.4866113
TGGTATC1218800.029.2810347
GGTATCA1230150.028.9356338
CGCAGAG1228400.028.80105416
GTATCAA1228750.028.6995779
GCAGTGG1257300.028.6452123
TATCAAC1234000.028.61010710
GAGTACA1224550.028.47365820
GCAGAGT1241800.028.39409417
GTACATG1228850.028.36200722
TACATGG1229900.028.20383323
AGTACAT1235250.028.1071721
CAGAGTA1262550.027.89925218
Produced by FastQC (version 0.11.5)