FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-rna-lib1.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-rna-lib1.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22469551
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258750.11515583911756848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT995450.037.2754361
AGCAGTG1073800.034.531522
GTGGTAT1243000.029.873286
CAGTGGT1254850.029.6429064
TCAACGC1233600.029.54806312
ATCAACG1236950.029.48131611
AGTGGTA1267800.029.3718225
CAACGCA1242650.029.3446213
TGGTATC1266050.029.2539447
GGTATCA1277600.028.9011658
GTATCAA1270550.028.7562249
CGCAGAG1279500.028.65448216
GCAGTGG1303350.028.6060713
TATCAAC1278650.028.5912510
GAGTACA1270850.028.345520
GCAGAGT1288150.028.29130717
GTACATG1277200.028.27501922
TACATGG1278250.028.07824523
AGTACAT1283350.028.01023521
CAGAGTA1303900.027.8560918
Produced by FastQC (version 0.11.5)