FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-rna-lib-1.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-rna-lib-1.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20843041
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238830.11458500705343333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT918650.037.419281
AGCAGTG991150.034.7108082
GTGGTAT1151850.029.8689736
CAGTGGT1160100.029.7017754
TCAACGC1146700.029.50332812
ATCAACG1148700.029.45683911
AGTGGTA1172250.029.417795
CAACGCA1151950.029.3582613
TGGTATC1175150.029.1866657
GGTATCA1184000.028.8335868
CGCAGAG1184150.028.70019716
GCAGTGG1207250.028.615713
GTATCAA1184650.028.5952639
TATCAAC1187700.028.53806310
GAGTACA1178850.028.40781420
GTACATG1183300.028.31589522
GCAGAGT1196950.028.30550617
TACATGG1184850.028.16396123
AGTACAT1188100.028.03608921
CAGAGTA1212600.027.87206518
Produced by FastQC (version 0.11.5)