FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-HTO2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-HTO2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length47
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGCATTTACACTAAGTGTATAATGCAAATGTAATAAAAGTAC19.090909090909092No Hit
CAGTAGACACGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19.090909090909092No Hit
CACTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA19.090909090909092No Hit
GGGGCCGCGAGCCGGGGGGCCGGGGGCCGGGGGGTGGGGGGGAGGGG19.090909090909092No Hit
CGAGCCACTATTTATCACACAAAACCCAAACCAAACACAAACAACAA19.090909090909092No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19.090909090909092No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAGGGG19.090909090909092No Hit
CAGTAGTCACGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAATATTT19.090909090909092No Hit
AGTGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19.090909090909092No Hit
GTCTGCTAAAGAGAAAGGAAAATTTGAAGATATGGCAAAAGCGGACA19.090909090909092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAA19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)