FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-HTO1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-HTO1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2394
Sequences flagged as poor quality0
Sequence length47
%GC14

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.5012531328320802No Hit
GAGGTTAGTGATGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.37593984962406013No Hit
TGCAATTACCCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.37593984962406013No Hit
ACTTCCGTCGATCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.37593984962406013No Hit
GAGGTTAGTGATGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.37593984962406013No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.2506265664160401No Hit
TGCAATTACCCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.20885547201336674No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1670843776106934No Hit
ATCTGTATGTCACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1670843776106934No Hit
GAGGTTAGTGATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1670843776106934No Hit
ATTGACCCGCGTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1670843776106934No Hit
TTCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12531328320802004No Hit
AAAGTGATGCCACTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12531328320802004No Hit
CCTTCCGTATCCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12531328320802004No Hit
AGCAATTACCCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12531328320802004No Hit
TGAGAACGACCCTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12531328320802004No Hit
AAAGTGATGCCACTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12531328320802004No Hit
CCATTCAACTTCCGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12531328320802004No Hit
ACTTCCGTCGATCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12531328320802004No Hit
ATTGACCCGCGTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12531328320802004No Hit
TAAGACTTGGCCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12531328320802004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA254.90058E-541.015
GAAAAAA452.0118023E-836.44444316
ACAAAAA606.031769E-930.75000215
CAAAAAA1100.026.09090816
TAAAAAA1003.0460797E-820.516
ATAAAAA855.34016E-619.29411715
AATAAAA655.173526E-418.92307714
Produced by FastQC (version 0.11.5)