FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-BulkC7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-BulkC7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16434406
Sequences flagged as poor quality0
Sequence length47
%GC17

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4990723.0367510696766282No Hit
TGCAATTACCCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3805372.3154898327326223No Hit
TGCAATTACCCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3543112.1559099854293486No Hit
GAGGTTAGTGATGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2295141.3965457589401162No Hit
AAAGTGATGCCACTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2144551.3049148232068746No Hit
AAAGTGATGCCACTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2095841.2752757842297433No Hit
ACTTCCGTCGATCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090821.2722212168787848No Hit
GAGGTTAGTGATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2061711.254508377120536No Hit
GAGGTTAGTGATGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1943641.1826651964178079No Hit
AAAGTGATGCCACTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1661501.0109887756210962No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1449000.8816868708245372No Hit
ACTTCCGTCGATCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1434980.8731559875057243No Hit
GCTGCGCTTTCCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1306610.795045467417563No Hit
ACTTCCGTCGATCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1039910.6327639709034814No Hit
GCTGCGCTTTCCATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA976350.5940890105793906No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA958610.5832945833271979No Hit
GCTGCGCTTTCCATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA859940.5232559059329556No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA685030.4168267474954678No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG623470.37936874627534456No Hit
ATCTGTATGTCACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA605700.36855606463659224No Hit
CAGCCCACTGCAATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA563830.34307902579503025No Hit
TGAAGGCTCATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA462070.281160146585158No Hit
ATCTGTATGTCACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA446100.27144272813997655No Hit
CTGGGCAATTACTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA433920.2640314471968138No Hit
ATCTGTATGTCACAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA415400.25276240589407367No Hit
AAAGTGATGCCACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA395140.24043461017088175No Hit
TGTACCCGCTTGTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA389500.23700278549769307No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA359820.21894311239481365No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA356830.21712375853438207No Hit
GCGCTACTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA348310.21193951275148004No Hit
CTGGGCAATTACTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA347250.21129452442637722No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA343620.209085743652676No Hit
CCTTCCGTATCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA343380.2089397085601999No Hit
TAAGACTTGGCCGTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA342290.2082764658485375No Hit
TGCAATTACCCGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA334150.20332344229538934No Hit
GCGCTACTTCCTTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA326890.19890588074798687No Hit
CACTTAACTCTGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA324200.19726907075315042No Hit
CAGTTCCTCAGTTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA318980.19409280749179497No Hit
CTGGGCAATTACTCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA317460.19316791857277957No Hit
GTGCATTCAACAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA317460.19316791857277957No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA307350.18701619030222327No Hit
CAGTTCCTCAGTTCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA306970.18678496807246942No Hit
TGAGAACGACCCTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA299940.18250735682202326No Hit
GCGCTACTTCCTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA296700.18053588307359572No Hit
CAGTTCCTCAGTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA288480.17553418115628883No Hit
CAGCCCACTGCAATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA273800.16660170133316654No Hit
TACGATTTGTCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA257680.15679301095518755No Hit
TCACGCAGTCCTCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA255380.1553935079856248No Hit
TGAAGGCTCATTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA255330.15536308400802562No Hit
AACTCCCTATGGAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252310.1535254757610345No Hit
GTGCATTCAACAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA249750.15196776810795595No Hit
TCGCTTGGATGGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA247740.15074472420846852No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA246290.14986242885809198No Hit
TGCAATTACCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA236730.14404536434112677No Hit
CAGCCCACTGCAATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA236010.14360725906369845No Hit
TGAAGGCTCATTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA234480.1426762853491632No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA234170.14248765668804822No Hit
CACTTAACTCTGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA231780.14103339055880693No Hit
TGTACCCGCTTGTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230220.14008416245771219No Hit
ATTTCCATTCCCTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA229520.13965822677132353No Hit
TGACCCGACCTTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA228690.13915318874317698No Hit
TCACGCAGTCCTCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA227940.13869682907918912No Hit
CCTTCCGTATCCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA227600.13848994603151463No Hit
GAGGTTAGTGATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA225530.13723039335890813No Hit
AAGTTCACTCTTTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA225210.13703567990227333No Hit
TAAGACTTGGCCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224900.13684705124115834No Hit
GTGCATTCAACAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA223410.13594041670870247No Hit
GATTAACCAGCTCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA222730.13552665061335348No Hit
CCTTCCGTATCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA217170.1321435043043235No Hit
TCCCTTGCGATTTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA217090.13209482594016478No Hit
TGACCCGACCTTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA216950.13200963880288707No Hit
TCACGCAGTCCTCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA213710.13003816505445953No Hit
TCGCTTGGATGGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212770.12946619427559475No Hit
CGATCCCTTACCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211830.12889422349672996No Hit
GTATAGACCAAAGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210460.12806060651051215No Hit
AACTCCCTATGGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210400.12802409773739312No Hit
TATTGGGACACTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210250.12793282580459556No Hit
TGCAATTACCCGGATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA209530.1274947205271672No Hit
TAAGACTTGGCCGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206020.12535895729970406No Hit
CCTTCCCTTTCCTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205000.12473830815668058No Hit
TGACCCGACCTTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA204830.12463486663284332No Hit
GATTAACCAGCTCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA203510.1238316736242247No Hit
CAGTCGTGGTAGATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA197130.119949574082568No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA194430.11830667929221172No Hit
TCGCTTGGATGGTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA194000.11804503308485868No Hit
TGAGAACGACCCTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193450.11771036933126758No Hit
CGATCCCTTACCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA191200.11634129033930403No Hit
TGTACCCGCTTGTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189320.11519734878157445No Hit
CCATTCAACTTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189050.11503305930253883No Hit
TACGATTTGTCAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA188410.1146436323892692No Hit
ATTCCTCATTCCTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA188050.11442457975055502No Hit
TCTCAGACCTCCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA186810.1136700651060951No Hit
TGTCCTACCCAACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185540.11289729607507568No Hit
GGACGCTTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA183800.11183854165462384No Hit
TCTCAGACCTCCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182470.11102926385048537No Hit
CATTAGAGTCTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182220.11087714396248943No Hit
CCATTCAACTTCCGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181500.11043903868506108No Hit
TGATCCGAAGTCGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181070.11017739247770805No Hit
ACTTCCGTCGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180590.10988532229275583No Hit
TACGATTTGTCAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180270.109690608836121No Hit
AACTCCCTATGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177550.10803554445472505No Hit
AAGTTCACTCTTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA176970.10768262631457443No Hit
TGCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT176800.10757918479073719No Hit
TATTGGGACACTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175890.10702546839843191No Hit
TGAGAACGACCCTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174560.10621619059429346No Hit
TGCAATTACCCGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171410.10429948000554447No Hit
AGCTGTAAGTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171260.10420820807274689No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170200.10356321974764407No Hit
CATTAGAGTCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169560.10317379283437442No Hit
ACCTTTATGCCACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169480.10312511447021572No Hit
TCCCTTGCGATTTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169200.10295474019566025No Hit
CGATCCCTTACCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168970.10281478989870399No Hit
ATCCTTCTCCCTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168790.1027052635793469No Hit
CCATTCAACTTCCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168450.1024983805316724No Hit
GCACTCCTGCATGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA165690.10081897696819708No Hit
GGACGCTTTCCTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA165450.10067294187572097No Hit
TCTCAGACCTCCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA165370.10062426351156227No Hit
TGCAATTACCCGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA164540.10011922548341572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAAC200.001111271841.00996413
TCGGAAT256.733116E-541.00996413
GCGACAT304.104624E-641.00971612
CTCGTAG1950.041.00971612
GCCTGAG256.7333574E-541.00971612
CGTACTA1250.041.00971612
TTCATAG600.041.00971612
GTGACAT506.002665E-1141.00971612
CGGTTAT401.549779E-841.00971212
GGACTGA352.5160807E-741.00971212
CGCCTGA401.549779E-841.00971212
ACGGCAC200.001111305141.00971212
TCTCGAG352.5160807E-741.00971212
CTCGCGT200.001111305141.00971212
GTCCCAT459.586074E-1041.00971212
TGGTCAT200.001111305141.00971212
CGGAATG304.1062576E-641.00734311
ATAGGCC256.735657E-541.00734311
CACGGCT600.041.00734311
TGCATCC256.735657E-541.00734311
Produced by FastQC (version 0.11.5)