FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-BulkB4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-BulkB4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15581672
Sequences flagged as poor quality0
Sequence length47
%GC17

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5360343.440157128195229No Hit
GTGCATTCAACAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4797403.0788736921172517No Hit
TGCAATTACCCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4103552.633574882079407No Hit
TGCAATTACCCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3822672.4533118140338215No Hit
GTGCATTCAACAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3726592.391649625277698No Hit
GTGCATTCAACAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3379542.1689199978025466No Hit
GAGGTTAGTGATGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1941801.2462077240491265No Hit
GAGGTTAGTGATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1728791.1095022408378254No Hit
GAGGTTAGTGATGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1647991.0576464451311771No Hit
ACTTCCGTCGATCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1465850.9407526997102751No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110440.7126577943625049No Hit
ACTTCCGTCGATCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980620.629341960220957No Hit
GCTGCGCTTTCCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA961540.6170968045021099No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA742950.4768101908447309No Hit
GCTGCGCTTTCCATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730280.4686788426813246No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG725340.46550845121114093No Hit
ACTTCCGTCGATCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA699700.4490532209893778No Hit
GCTGCGCTTTCCATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA639250.4102576411568669No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA532680.3418631838739771No Hit
ACCTTTATGCCACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA425570.2731221655801765No Hit
ACCTTTATGCCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA407690.2616471454411311No Hit
ACCTTTATGCCACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400750.2571931946712779No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA373510.23971111700977918No Hit
CAGCCCACTGCAATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA356580.22884578753807677No Hit
GTGCATTCAACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA352440.2261888197877609No Hit
TGCAATTACCCGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA348420.2236088655954252No Hit
CTGGGCAATTACTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA337450.21656854283673793No Hit
ATCTGTATGTCACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA336250.21579840725693625No Hit
TGTACCCGCTTGTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA322450.20694184808921662No Hit
CTCATTGTAACTCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA318860.20463785914630983No Hit
TATCCCTTGGGATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA313230.20102463971774018No Hit
GCGCTACTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA311350.19981809397605085No Hit
TGAAGGCTCATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA307250.19718679741172834No Hit
CTCATTGTAACTCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA304420.195370561002696No Hit
CAGTTCCTCAGTTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA302260.19398431695905294No Hit
CAGTTCCTCAGTTCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA297170.19071765854139403No Hit
GCGCTACTTCCTTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA296110.1900373721125692No Hit
GATTAACCAGCTCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA288790.18533954507577877No Hit
CCTTCCGTATCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA279790.17956352822726598No Hit
CTGGGCAATTACTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA274880.17641239014657734No Hit
CAGTTCCTCAGTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270270.17345378596083913No Hit
GCGCTACTTCCTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA264760.16991758009024963No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA263470.16908968434196278No Hit
TACGATTTGTCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA261870.16806283690222718No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA261470.16780612504229328No Hit
GATTAACCAGCTCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260370.16710016742747505No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA255130.16373724206234094No Hit
TATTGGGACACTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA249070.15984805738434232No Hit
ATCTGTATGTCACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA249060.15984163958784398No Hit
TGCAATTACCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248610.15955283874541834No Hit
CTGGGCAATTACTCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248440.15944373620494642No Hit
CTCATTGTAACTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248260.1593282158679762No Hit
TATCCCTTGGGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA242490.15562514728842963No Hit
AATCCTTCCGAATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239380.15362921257744355No Hit
TGAGAACGACCCTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239370.1536227947809452No Hit
ATCTGTATGTCACAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA236190.1515819354944707No Hit
TGCAATTACCCGGATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA234760.15066419059520697No Hit
CACTTAACTCTGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA231450.14853989995425393No Hit
AACTCCCTATGGAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA229190.1470894779456274No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA229060.14700604659114888No Hit
ATTTCCATTCCCTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA227480.14599203474440997No Hit
AATCCTTCCGAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA217120.1393431975721219No Hit
TATTGGGACACTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA215710.13843828826585491No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA215120.1380596382724524No Hit
TAAGACTTGGCCGTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA201550.12935068842419478No Hit
TCGCTTGGATGGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200860.1289078604658088No Hit
GATTAACCAGCTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199790.1282211562404856No Hit
TATCCCTTGGGATGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA197900.12700819270229793No Hit
TGTACCCGCTTGTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA194910.125089271549292No Hit
TATTGGGACACTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193550.12421645122551675No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA192810.12374153428463903No Hit
TCTCAGACCTCCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA191550.12293289192584723No Hit
AACTCCCTATGGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190430.12221409871803232No Hit
TACGATTTGTCAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189710.12175201737015129No Hit
TCACGCAGTCCTCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189350.12152097669621079No Hit
AATCCTTCCGAATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189200.12142470974873558No Hit
GTGCATTCAACAGTACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185460.11902445385835358No Hit
TACGATTTGTCAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA184960.11870356403343621No Hit
CCATTCAACTTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA184250.11824790048205353No Hit
CCTTCCGTATCCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA183670.11787566828514937No Hit
TGCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT183050.11747776490225184No Hit
TCCCTTGCGATTTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182500.11712478609484271No Hit
GAGGTTAGTGATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182200.11693225219989228No Hit
TCTCAGACCTCCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181580.11653434881699473No Hit
TGCAATTACCCGGATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA179310.11507750901186985No Hit
CAGCCCACTGCAATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177600.11398006581065243No Hit
CATTAGAGTCTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177370.11383245649119042No Hit
TGCAATTACCCGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177330.11380678530519701No Hit
GCACTCCTGCATGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA176330.11316500565536228No Hit
CACTTAACTCTGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174660.11209323364013823No Hit
CCTTCCGTATCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA173730.11149637856579192No Hit
TCGCTTGGATGGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA173530.11136802263582496No Hit
TGCAATTACCCGGATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171570.11011013452214885No Hit
CCATTCAACTTCCGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170880.10966730656376286No Hit
TCTCAGACCTCCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168850.1083644938745983No Hit
GTATAGACCAAAGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168460.10811419981116276No Hit
GGACGCTTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168300.1080115150671892No Hit
TGCAATTACCCGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167050.10720929050489576No Hit
GTGCATTCAACAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166620.10693332525546681No Hit
CCATTCAACTTCCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162910.10455232275457986No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162630.10437262445262614No Hit
CATTAGAGTCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162470.10426993970865257No Hit
TGAAGGCTCATTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162150.10406457022070546No Hit
TGTACCCGCTTGTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160980.1033136880303988No Hit
TCACGCAGTCCTCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA159570.10240877872413179No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA159430.10231892957315492No Hit
TGCAATTACCCGGATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156900.100695227059073No Hit
TGAGAACGACCCTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156640.10052836435011595No Hit
AACTCCCTATGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156470.10041926180964404No Hit
CCTTCCCTTTCCTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156320.10032299486216883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCTA200.001111128141.0110314
TCGCAAG200.001111145741.010913
ATTTTCT200.001111145741.010913
TGTTAAG650.041.010913
GGTGAAT200.001111145741.010913
AGTGTCA200.001111145741.010913
TCGCAAT304.1037983E-641.01089513
CGGTTAT352.515626E-741.01063512
TGGCGAC352.515626E-741.01063512
GTAGGAT553.6379788E-1241.01063512
GACCCAT200.001111180941.01063512
CTCGCAT1400.041.01063512
GAGTTAT700.041.01063512
CGGTCTA553.6379788E-1241.01063512
GTGACAT401.5494152E-841.01063512
TTTCTAG800.041.01063512
TGGTCAG200.001111180941.01063512
TGCACAT553.6379788E-1241.01063512
GTTGCAG800.041.01063512
GCGACTA256.732454E-541.0106312
Produced by FastQC (version 0.11.5)