FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-ADT2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-ADT2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50349199
Sequences flagged as poor quality0
Sequence length47
%GC17

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14931572.9656022928984433No Hit
TGCAATTACCCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12750392.532391826134116No Hit
TGCAATTACCCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8020001.5928753901328203No Hit
TGAGAACGACCCTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7470951.483826982034014No Hit
ACTTCCGTCGATCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7193361.4286940294720478No Hit
GAGGTTAGTGATGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6360571.2632911995283183No Hit
GAGGTTAGTGATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5623401.116879734273429No Hit
TGAGAACGACCCTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5115041.0159128847312944No Hit
TGAGAACGACCCTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4914820.9761466115876045No Hit
GAGGTTAGTGATGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4779370.9492444954288151No Hit
ACTTCCGTCGATCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4197490.8336756261008244No Hit
AGCTGTAAGTTTCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3939320.7823997358925213No Hit
AGCTGTAAGTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3916320.7778316393871529No Hit
ACTTCCGTCGATCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3897100.7740142996117972No Hit
GCTGCGCTTTCCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3855630.7657778229997264No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3575540.7101483382089157No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3475500.6902791045394784No Hit
TTCCTGCACGAGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2780880.5523186178195207No Hit
GCTGCGCTTTCCATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2675530.531394749696018No Hit
TTCCTGCACGAGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2245480.4459812756902051No Hit
TTCCTGCACGAGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2215800.44008644506936445No Hit
AATCCTTCCGAATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2045810.406324239636861No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2029160.40301733499275727No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1817590.3609968055301138No Hit
AGCTGTAAGTTTCGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1662550.33020386282609976No Hit
AATCCTTCCGAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1648860.3274848523409479No Hit
GCTGCGCTTTCCATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1563110.3104537968915851No Hit
ATCTGTATGTCACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1479610.2938696204481823No Hit
TGAAGGCTCATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1442170.28643355378900864No Hit
GCGCTACTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1426600.28334115106776575No Hit
ACCTTTATGCCACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1315100.2611958136613057No Hit
AATCCTTCCGAATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1288220.25585709913677074No Hit
GATTAACCAGCTCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1275860.2534022437973641No Hit
GATTAACCAGCTCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1271110.25245883256255974No Hit
GCGCTACTTCCTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1192960.23693723508888395No Hit
GCGCTACTTCCTTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1161760.2307405128729059No Hit
CCTTCCGTATCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1147460.22790034852391594No Hit
CAGCCCACTGCAATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090140.21651585758097164No Hit
TGAGAACGACCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1072740.21305999326821468No Hit
ATCTGTATGTCACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1067100.2119398165599417No Hit
TATCCCTTGGGATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1066440.21180873205152678No Hit
ACCTTTATGCCACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060900.2107084166324076No Hit
GATTAACCAGCTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1046350.20781859906053324No Hit
ACCTTTATGCCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1041050.20676595073538312No Hit
CACTTAACTCTGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1032420.20505192148141224No Hit
TGAAGGCTCATTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1026880.20395160606229307No Hit
TAAGACTTGGCCGTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020750.2027341090371666No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1014970.20158612652407837No Hit
ACTCTTTCGTTTACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA975720.19379057053122137No Hit
TAAGACTTGGCCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA967280.1921142777266427No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA947380.1881618811850413No Hit
ATCTGTATGTCACAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA924680.18365336854713418No Hit
TGTACCCGCTTGTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA917290.18218561927867014No Hit
TGACCTTCCTCTCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA916980.18212404928229345No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA899370.17862647626231354No Hit
TCGCTTGGATGGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA896860.17812795790455377No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA872880.1733652207654783No Hit
GGACGCTTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA849040.16863028943121816No Hit
CACTTAACTCTGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA844730.16777426786869043No Hit
TGAAGGCTCATTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA842140.1672598604796076No Hit
AGCTGTAAGTTTCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830440.16493608964861586No Hit
CGTGTTTAACTTGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA823590.16357559134158223No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820180.16289832138143848No Hit
TGTCCTACCCAACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA813080.1614881698515204No Hit
CTTCTCCTCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA811900.16125380663950584No Hit
AACTCCCTATGGAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA809210.16071953796126925No Hit
CATTTGTCTGCCGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA801010.15909091225065963No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770740.15307890002381172No Hit
GTATAGACCAAAGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA758690.15068561468078173No Hit
TAAGACTTGGCCGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA753500.14965481377370074No Hit
CCTTCCGTATCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750140.14898747445813387No Hit
AGCTGTAAGTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA747190.14840156642809751No Hit
CCTTCCGTATCCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA739670.14690799748373354No Hit
GGACGCTTTCCTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA732890.14556140207910756No Hit
ACTCTTTCGTTTACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA713740.1417579652061595No Hit
TATCCCTTGGGATGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA704780.13997839369798118No Hit
TGGCTTCAGGTCCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA694820.13800020929826512No Hit
TGACCTTCCTCTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA693790.1377956380199812No Hit
TGAGAACGACCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA692890.13761688641759723No Hit
GCGCAACTTGATGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA692660.13757120545254353No Hit
TCCCTTGCGATTTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA689120.13686811581649988No Hit
CCTTCCCTTTCCTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA681620.1353785191299667No Hit
GCGCAACTTGATGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680940.13524346236372103No Hit
ATCAGATGCCCTCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA674110.13388693631451815No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670040.13307858184595944No Hit
TATCCCTTGGGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA663810.13184122353167924No Hit
CCAGCTCATTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA662070.13149563710040352No Hit
GTATAGACCAAAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA658960.1308779510077211No Hit
ATCAGATGCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA658040.13069522714750637No Hit
CGTGTTTAACTTGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA632240.1255710145458322No Hit
TATTGGGACACTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630380.125201594567572No Hit
TCGCTTGGATGGTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620830.12330484145338638No Hit
TACGATTTGTCAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620120.12316382630039456No Hit
CTGGGCAATTACTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA618060.1227546837438268No Hit
GGACGCTTTCCTTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA609410.12103668223202518No Hit
TCGCTTGGATGGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA604850.12013100744661301No Hit
CGATCCCTTACCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA604250.12001183971169034No Hit
CCTTCCCTTTCCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA597960.11876256462391786No Hit
TCCCTTGCGATTTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA588370.11685786699407075No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA587870.11675856054830186No Hit
AACTCCCTATGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA586770.11654008636761033No Hit
TCCCTTGCGATTTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA586270.11644077992184146No Hit
TGTACCCGCTTGTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA584780.11614484671345018No Hit
TGACCTTCCTCTCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA576010.11440301165466407No Hit
AACTCCCTATGGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA573580.11392038232822732No Hit
CGATCCCTTACCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA573510.11390647942581968No Hit
TGTACCCGCTTGTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA573090.1138230620113738No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA572440.11369396363187427No Hit
TGACCCGACCTTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA572040.11361451847525916No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570510.11331064075120638No Hit
CAGTCGTGGTAGATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA569640.11313784753556853No Hit
ATCAGATGCCCTCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA558010.11082797960698441No Hit
TCCTTGGATGTACCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA557110.11064922800460043No Hit
ATTCCTCATTCCTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA552870.10980710934448033No Hit
GTATAGACCAAAGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA552120.10965814967582703No Hit
ACTTCCGTCGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA545140.10827183169289346No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA543700.10798582912907909No Hit
ATCCTTCTCCCTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA542730.10779317462428746No Hit
TACGATTTGTCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA541830.10761442302190348No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA537260.10670676210757593No Hit
GGGTTGTTGTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA535320.10632145309799268No Hit
TACGATTTGTCAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA532110.10568390571615648No Hit
CTCATTGTAACTCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA529990.10526284638609643No Hit
GCGCAACTTGATGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA527480.10476432802833666No Hit
ATCCTTCTCCCTTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA525170.10430553224888445No Hit
AGCTTACCTGCACGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524200.10411287774409281No Hit
TCACGCAGTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA522120.10369976292969427No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA522100.1036957906718635No Hit
CTCATTGTAACTCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA519400.10315953586471156No Hit
GCTCATTACCCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA517100.10270272621417473No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA516430.10256965557684443No Hit
CGATCCCTTACCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA513630.1020135394805387No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA508170.10092911309274254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCT200.001111622741.00749611
CTTGCTG200.001111622741.00749611
GTTTTCG850.041.005999
GGTCTAC256.7371686E-541.0059859
CATCTAT600.041.00554310
CCTGATC3300.041.00554310
GCTGATG256.737603E-541.0055410
GCTCTTG401.5512342E-841.0055410
GCTCTAG256.737603E-541.0055410
CGCTCGC200.001111883841.0055410
GGCATGC352.518227E-741.0055410
CTCCTAG459.604264E-1041.0055410
CTCCCGG1550.041.0046848
CTAGCCT401.551598E-841.0046848
CGCTGGT200.001112286441.002527
CTAGGTC200.001112286441.002527
GCCTCTC256.740527E-541.0025187
GACTGAC1000.041.0025187
TGAGCTG256.740527E-541.0025187
TGGATCG900.041.00116
Produced by FastQC (version 0.11.5)