FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n02_b-PBMCpilot-ADT1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n02_b-PBMCpilot-ADT1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52304648
Sequences flagged as poor quality0
Sequence length47
%GC17

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16347803.1254966097850425No Hit
TGCAATTACCCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14188592.7126824369413596No Hit
TGCAATTACCCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8809061.6841830194517322No Hit
ACTTCCGTCGATCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7845151.499895382146535No Hit
GAGGTTAGTGATGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7317031.3989253880458197No Hit
GAGGTTAGTGATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6487071.2402473294533978No Hit
GAGGTTAGTGATGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5486671.0489832567078934No Hit
ACTTCCGTCGATCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4651750.8893569076308475No Hit
ACTTCCGTCGATCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4328400.8275363979124761No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4157110.7947878743013432No Hit
GCTGCGCTTTCCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4073840.7788676830403294No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4018830.7683504532904992No Hit
GCTGCGCTTTCCATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2869900.5486892866576599No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2566900.49075944455261417No Hit
AATCCTTCCGAATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2351500.44957763600665085No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2334390.44630641620989403No Hit
AATCCTTCCGAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1938360.3705903918902198No Hit
ATCTGTATGTCACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1935590.370060802244573No Hit
TGAGAACGACCCTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1753650.3352761307178666No Hit
GCGCTACTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1721520.3291332732035593No Hit
CAGCCCACTGCAATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1688180.32275907869602716No Hit
TGAAGGCTCATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1665610.3184439746157932No Hit
GCTGCGCTTTCCATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1647330.31494906532971984No Hit
AATCCTTCCGAATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1549980.29633695269299964No Hit
ACCTTTATGCCACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1544430.29527586152572904No Hit
GATTAACCAGCTCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1512270.2891272683834905No Hit
GATTAACCAGCTCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1447790.27679949208338045No Hit
GCGCTACTTCCTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1436310.2746046584617107No Hit
GCGCTACTTCCTTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1408470.2692819957415639No Hit
CCTTCCGTATCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1351720.25843209957172447No Hit
ATCTGTATGTCACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1346580.2574493953195135No Hit
TGAGAACGACCCTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1334300.25510161161967865No Hit
ACCTTTATGCCACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1291560.24693025369370616No Hit
ACCTTTATGCCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1236480.23639964081203646No Hit
ATCTGTATGTCACAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1234620.23604403188030249No Hit
TATCCCTTGGGATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1211630.23164862900903185No Hit
GATTAACCAGCTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210740.23147847204707314No Hit
TGAGAACGACCCTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210690.23146891266718783No Hit
TAAGACTTGGCCGTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1201280.22966983737277039No Hit
CACTTAACTCTGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1165690.22286547077039884No Hit
TGAAGGCTCATTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1148000.21948336216697223No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130970.21622743737803188No Hit
TGTACCCGCTTGTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1081840.20683439070271536No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1079390.20636598108833465No Hit
TAAGACTTGGCCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1067430.2040793774197658No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1053870.20148687359486675No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1036590.1981831519064998No Hit
TGACCTTCCTCTCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1033240.19754267345418325No Hit
GTATAGACCAAAGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1011530.19339199070797686No Hit
TCGCTTGGATGGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1009130.19293314047348142No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA989780.1892336604578622No Hit
AACTCCCTATGGAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970910.185625950489142No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA965000.18449603178669705No Hit
TGAAGGCTCATTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA951440.18190352796179796No Hit
ACTCTTTCGTTTACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940340.1797813456272567No Hit
CACTTAACTCTGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA939600.17963986680495395No Hit
CGTGTTTAACTTGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA923000.17646615268302732No Hit
TAAGACTTGGCCGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA915590.17504945258402274No Hit
GGACGCTTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA907700.17354098243811908No Hit
CCTTCCGTATCCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA894510.17101921802437137No Hit
CCTTCCGTATCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA894280.17097524487689889No Hit
TGTCCTACCCAACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA871800.16667734768045853No Hit
GTATAGACCAAAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA844730.1615018994105457No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA821520.15706443526777963No Hit
TATCCCTTGGGATGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA805270.15395763680505029No Hit
TATCCCTTGGGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790350.1511051178472705No Hit
TCCCTTGCGATTTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA789590.15095981527301358No Hit
GGACGCTTTCCTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA784710.15002681979620627No Hit
CATTTGTCTGCCGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA775860.1483348095565044No Hit
TGACCCGACCTTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA774620.14809773693534845No Hit
CCTTCCCTTTCCTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA774530.1480805300515549No Hit
TGACCTTCCTCTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770770.14736166468417874No Hit
TGGCTTCAGGTCCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA763410.14595452396505948No Hit
GCGCAACTTGATGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA755450.14443267068731636No Hit
GCGCAACTTGATGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA745920.14261065288117414No Hit
TATTGGGACACTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA744710.1423793158879494No Hit
CAGCCCACTGCAATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA737070.14091864264147233No Hit
CGTGTTTAACTTGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA733480.14023227916570627No Hit
GTATAGACCAAAGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA728740.13932604995257783No Hit
AACTCCCTATGGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA727590.13910618421521545No Hit
CTTCTCCTCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA726370.1388729353460136No Hit
ATCAGATGCCCTCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA724190.1384561463830136No Hit
TCGCTTGGATGGTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA714900.13668001360032095No Hit
AACTCCCTATGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA708310.13542008733143562No Hit
ACTCTTTCGTTTACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA707560.13527669663315584No Hit
CTGGGCAATTACTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA696490.1331602499265457No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690790.13207048061961912No Hit
TCCCTTGCGATTTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA685710.1310992476232705No Hit
CAGTCGTGGTAGATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA685250.13101130132832556No Hit
CCAGCTCATTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA684260.1308220256065962No Hit
TCGCTTGGATGGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA683850.13074363869153655No Hit
AGCTGTAAGTTTCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA677080.12944929865506408No Hit
ATTCCTCATTCCTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA673030.12867498888435308No Hit
TACGATTTGTCAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA671210.12832702745652738No Hit
TGTACCCGCTTGTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA669460.12799244916054114No Hit
CAGCCCACTGCAATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA668090.12773052215168335No Hit
TCCCTTGCGATTTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA664380.12702121616419254No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA663250.12680517417878426No Hit
ATCAGATGCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA662520.12666560723245857No Hit
TACGATTTGTCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA659710.12612837008290353No Hit
CGATCCCTTACCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA656980.125606427941165No Hit
GGACGCTTTCCTTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA654090.12505389578379345No Hit
TGACCTTCCTCTCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA654060.12504816015586226No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA649830.12423943661756408No Hit
TGTACCCGCTTGTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA644540.12322805422569712No Hit
GTGCATTCAACAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA641200.12258948764935766No Hit
GGGTTGTTGTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA637630.12190694792554573No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA628890.12023596832159161No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA627260.11992433253733015No Hit
TTCCTGCACGAGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA627200.11991286128146775No Hit
CTCATTGTAACTCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA619490.11843880490315126No Hit
GCTCATTACCCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA614620.11750772130232098No Hit
CGATCCCTTACCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610940.11680415094276135No Hit
TGACCCGACCTTTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA608640.11636441946803658No Hit
CTCATTGTAACTCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA602660.11522111763375216No Hit
TACGATTTGTCAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600900.11488462746178886No Hit
TCCTTGGATGTACCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600710.11484830181822464No Hit
AGCTGTAAGTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600570.11482153555454575No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA593820.1135310192700274No Hit
CCTTCCCTTTCCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA592660.11330924165668796No Hit
CAGTCGTGGTAGATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA589130.11263434943678428No Hit
CGATCCCTTACCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA583490.11155605138572006No Hit
ATCAGATGCCCTCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA579680.11082762663845859No Hit
TTCCTGCACGAGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA577720.11045289894695402No Hit
ACTTCCGTCGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA577260.11036495265200905No Hit
TGACCCGACCTTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA577060.11032671513246776No Hit
CATTAGAGTCTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA575920.11010876127108245No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA571740.1093095971126696No Hit
GCGCAACTTGATGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA569420.10886604188599071No Hit
AGCTTACCTGCACGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA568530.10869588492403198No Hit
TCACGCAGTCCTCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA566110.10823321093758245No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA564800.10798275518458704No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA564740.10797128392872465No Hit
CCAGCTCATTAGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560770.10721226916583015No Hit
TCACGCAGTCCTCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA558610.10679930395478428No Hit
ATCCTTCTCCCTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA556700.10643413564316502No Hit
TCACGCAGTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA549180.10499640490841272No Hit
GTGCATTCAACAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA546030.10439416397563751No Hit
TTTCAACGCCCTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA543720.10395252062493567No Hit
GGATAACCGCGCTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA539770.10319732961399529No Hit
TGATCCGAAGTCGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA538610.10297555200065585No Hit
TGTCCTACCCAACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA537390.10274230313145401No Hit
CGTGTTTAACTTGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA536840.10263714995271547No Hit
CATTAGAGTCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA534310.10215344533051823No Hit
TCACGCAGTCCTCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530430.10141163745141732No Hit
CAGTTCCTCAGTTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA529620.10125677549727512No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA526950.10074630461139897No Hit
TGCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT525250.10042128569529805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCGG256.7334266E-541.00985312
AGGATCC1200.041.0069111
TTAATCG800.041.0060849
GGCCATC950.041.0060849
ATATCTT256.737077E-541.0060849
TGATGCT401.5510523E-841.0060849
TCGCGTC200.001111811541.0060849
TGCTGTT304.1072963E-641.006089
GCGGATC1500.041.00498610
CCACGTC459.604264E-1041.00498610
CGAACTG200.001111958141.00498210
GGATCGC304.108053E-641.00498210
CGCGTCT200.001111958141.00498210
AGGACTC401.551598E-841.004718
GTCCCCT650.041.004718
TTCGCGT200.001111994741.004718
GGCTCTA950.041.004718
GACTATG256.7384084E-541.004718
GAGTACT200.001112387641.0017667
GCTCTGG459.604264E-1041.0017667
Produced by FastQC (version 0.11.5)