FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38845851
Sequences flagged as poor quality0
Sequence length28
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG1683600.4334053590433634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATCC83900.019.6257554
CGATATC83950.019.4975013
GCGATAT85450.019.1958832
GTCAAGA113400.018.7689046
ATATCCC87050.018.6883245
CCCTCTA90250.018.6595029
TATCCCT91600.018.2523636
CACGTCA116350.018.209023
CCACGTC115300.018.0046672
TCCCTCT100450.017.7940338
CAAGAAT129400.017.3747128
CCCGGAA25250.017.1783351
AACGACC26600.017.039552
ATCCCTC101550.016.9513937
TCCACGT129750.016.9016151
CTGCACC47800.016.8674019
TGCACCT50100.016.07109510
AAGAGAC47750.015.8484237
TCTCTGC53550.015.5902586
GTCTCTG52100.015.1585525
Produced by FastQC (version 0.11.5)