FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-rna-lic-1.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-rna-lic-1.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20843041
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA72450.019.6540411
CGGAGAA47150.018.1107311
CCGGAAG46850.018.0326522
GCGCAGG40500.018.0069185
AGGGCCT65750.017.8911341
TATGAGA43100.017.737389
ATGGGAG38450.017.6581331
AACGAAA57650.017.5035441
AAAAGAT30300.017.3889665
GATGAGG47700.017.1867541
AAACGAG25700.016.9489969
GTATGAG52250.016.9469098
ATCGGCG41500.016.9437181
GCGGAAA38400.016.8500841
AGGGAAG30300.016.844753
CAAAAGA28450.016.7410374
TGTATGA52350.016.7044187
TGACTCC47450.016.6975291
TAGACGC38850.016.676517
GCCCAGA50750.016.6308821
Produced by FastQC (version 0.11.5)