FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-rna-lib2.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-rna-lib2.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19815505
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGAA39600.017.6167531
ATGGGAG38850.017.5321
TGAGGAG43050.017.4829521
CGCATAA78250.017.3806461
TAACGAC43250.017.3512211
GACACGC54550.017.3474451
TCGAGAA39250.017.3192168
GGAGAAG32500.017.2954243
CCGAAGC37400.017.2943762
CCCGGAA36100.017.2825411
CTGGACG38650.017.1956751
ATGAGCC39200.017.0607455
GATGAGG37750.017.0518191
GGGATAG31050.016.9694443
CACGGAG52700.016.9488053
GAAATAG46100.016.9176083
GACGGCA32150.016.7652023
GAACCGA26800.016.6636710
CCCGAAG47550.016.6614881
GCGAACA38200.016.61467610
Produced by FastQC (version 0.11.5)