FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-rna-lib2.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-rna-lib2.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21514571
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGCA35000.017.8196893
CGCATAA79750.017.7160871
CTGGACG39200.017.628161
CCCGGAA39250.017.4094641
ATGGGAG43600.017.3634261
TGAGGAG44850.017.2965771
CCCAAGA43350.017.2289854
ATGAGCC43000.017.1902355
CGACGGC36400.017.1347122
AAACGAA41400.017.116661
GCTACCT78500.017.11511
CACGGAG55450.017.0799523
GAAGGGC28700.017.0169646
TCGAGAA40500.017.0020148
CCGAAGC43700.016.9909462
CGCGTGA43950.016.9747851
GACACGC61450.016.9395851
CGCCAGA36950.016.9148181
GGAGAAG38750.016.8900263
CGGCATG50900.016.8561295
Produced by FastQC (version 0.11.5)