FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-rna-lib2.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-rna-lib2.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25203469
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG50500.017.7807791
ATGAGCC51450.017.3815755
CGCATAA95700.017.3741871
CGCGTGA54800.017.2088681
CACGGAG69550.017.2072833
CTGGACG47650.017.1815381
TAACGAC51950.017.1150341
TCGAGAA46950.017.0560678
AAACGAA47800.017.0355361
GACACGC69600.017.0277961
TGAGGAG55150.016.9998871
GAAATAG63350.016.981373
CCGAAGC45950.016.9489542
GACGGCA39950.016.9056573
GGGATAG41250.016.8528613
CCATGAC61200.016.8052187
CCCAAGA51000.016.7367524
GCTACCT94300.016.7102641
ACAGAAA81250.016.6448731
CCCGAAG59050.016.6225241
Produced by FastQC (version 0.11.5)