FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-rna-lib2.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-rna-lib2.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20148510
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGAA36850.017.8502868
CGCATAA76450.017.8060571
CTGGACG37950.017.8045961
CACGGAG52550.017.7298533
ATGGGAG40900.017.4890251
GACGGCA32650.017.4855233
CCGAAGC37500.017.3365862
ATGAGCC40450.017.2677445
AAACGAA36950.017.1845021
TAACGAC41750.017.0802441
GCTACCT72400.017.054141
GAAATAG49450.016.9727693
CCATGAC45650.016.9393237
CGCGTGA44700.016.937781
GACACGC55650.016.9073871
CCCGGAA38900.016.8888591
CCCGAAG48200.016.735261
GAAGGGC26950.016.6525926
CCCAAGA37550.016.6093434
AGCTCAA56100.016.5952221
Produced by FastQC (version 0.11.5)