FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-rna-lib1.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-rna-lib1.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20358161
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA72450.019.8518981
CGGAGAA45250.018.1419961
CCGGAAG50000.017.864632
AGGGCCT63450.017.8289741
GCGCAGG40600.017.556025
GATGAGG48950.017.4451161
ATGGGAG36850.017.4397471
AACGAAA56050.017.1789761
TATGAGA43200.017.0595729
TCCGGGA48150.016.9579141
ATCGGCG39300.016.9405571
GCGGAAA39100.016.8583431
AAACGAG25150.016.8383629
TCGACGG49800.016.8381
CAAAAGA27150.016.8133934
TGGGAAG34350.016.7868581
TGACTCC49600.016.7062171
GCCCAGA49950.016.699311
TATATCC64950.016.6209071
GTATGAG52100.016.5944148
Produced by FastQC (version 0.11.5)