FastQCFastQC Report
Wed 5 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-rna-lib1.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-rna-lib1.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21411031
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA74150.019.8112981
CGGAGAA49900.018.302921
CCGGAAG48600.018.1072922
GCGCAGG41700.017.7262065
GATGAGG51950.017.7077561
AACGAAA59450.017.6023851
AGGGCCT68750.017.590081
ATCGGCG45600.017.5433431
CCCCAAG30900.017.4785565
TATGAGA44150.017.190999
GGCGAGT24150.017.1713224
ATGGGAG42600.017.0998061
GCCCAGA54500.017.0407521
ACCCCAA34650.016.8883764
TCCGGGA48450.016.874771
AAACGAG27050.016.7944349
CAAGACT71500.016.7288571
AATGGCT59400.016.7094781
CGTCAGG62850.016.6438874
TCGACGG53850.016.6334191
Produced by FastQC (version 0.11.5)