FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-rna-lib1.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-rna-lib1.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22469551
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA80650.019.839171
CGGAGAA53900.018.599151
AGGGCCT69400.018.044561
CCGGAAG53350.018.0416282
GCGCAGG44200.017.6692395
ATCGGCG47150.017.6442661
ATGGGAG44500.017.6069241
AACGAAA62250.017.5538921
TATGAGA47850.017.0800699
GATGAGG51750.017.0753141
GCGAAGA26500.017.01851710
CGGCGCA37100.016.8111613
TCCGGGA47850.016.7652131
GGCGAGT23300.016.759054
GCCCAGA54600.016.6677281
GGCGCAG24900.016.6540154
AAAAGAT31350.016.5960755
GTATGAG56950.016.55278
AGGGAAG31500.016.4474643
TCGACGG59450.016.4370781
Produced by FastQC (version 0.11.5)