FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-HTO2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-HTO2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40371593
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAAAG95800.017.4758433
ACAGAAA190750.017.4465941
TGAGGAG97450.017.3687271
AAACGAA103450.016.9783651
ACCAAAC130750.016.875871
AAACCCA112400.016.6936591
CTGGACG80000.016.6314371
CCCGGAA71950.016.626171
CAGAAAA94300.016.448042
GCCCGAA45800.016.339431
GATGAGG77200.016.2510341
GAAATAG89950.016.1177063
GAACCGA54700.016.06724710
GGAACCC63100.016.0628911
TCGAGAA72900.016.0392678
CTCTGCG100150.015.89265257
GATGACT99150.015.8832861
GACACGC109900.015.8617451
CGCCAGA87000.015.7992561
GATAAGG72900.015.7525244
Produced by FastQC (version 0.11.5)