FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-HTO1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-HTO1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42682995
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA97750.018.5314541
ATGGGAG101400.018.0923141
CGGAGAA103650.017.5509261
GCGAGAA128100.017.4742411
CGAAAAG90000.017.061933
AACGAAA189850.016.67731
GCGGAAA63700.016.3090651
AATGGCT105900.016.253141
TAACGAC113900.016.2323741
ACCTACC101750.016.1805651
GATGAGG90100.016.1352041
CAAAAGA60700.016.0736354
GCCGAGA46050.016.0285022
CGGGAAG69200.016.0228483
GCCCGAA51650.015.98039151
ACCCCAA55550.015.7816874
CGGGACT105400.015.766411
TACCCCA64200.015.7458373
TCCGGGA103100.015.74453451
GAGAAGC96800.015.7161762
Produced by FastQC (version 0.11.5)