FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-BulkC7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-BulkC7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16434406
Sequences flagged as poor quality0
Sequence length28
%GC17

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTTT156700.016.0885757
GAGCTTT90750.015.865917
AGGTTTT529650.015.8476348
GTGCTTT113350.015.8466197
GAGATTT94150.015.8420487
GGGATTT136200.015.8289077
GTGATTT113500.015.8159867
GACGTTT85600.015.7282217
AGCTTTT335600.015.5847758
GGGCTTT142850.015.5694367
CAGGTTT123150.015.5055537
AGTGCTT28050.015.4894586
AAGGTTT127450.015.474357
AGAGATT23900.015.4636986
AGATTTT345700.015.4189938
CGACGTT18700.015.411036
CGAGCTT21700.015.3587146
GCTTTTT1353000.015.3336029
GATTTTT1389150.015.3186079
GGTTTTT2252850.015.3140579
Produced by FastQC (version 0.11.5)