FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-BulkB4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-BulkB4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15581672
Sequences flagged as poor quality0
Sequence length28
%GC17

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC9750.016.5847520
GAGGTTT148000.016.201957
CGTGTAG751.8426726E-716.1325864
GAGCTTT89100.015.9992597
GTGATTT106250.015.9220857
AGGTTTT496600.015.8568968
GAGATTT87700.015.7028067
GACGTTT82350.015.6544047
CAGTCAC10450.015.57887418
GGGATTT134400.015.5744267
AGAGGTT33850.015.5650096
TGAGGTT38700.015.5186616
TGATTTT356000.015.4549028
AAGGTTT124500.015.4522947
GGGGATT44850.015.4507886
GCGATTT79400.015.4463877
GGGGTTT236150.015.4407417
CAGGTTT109500.015.4294217
GAAGTTT104050.015.3707437
CGAGCTT20200.015.3557236
Produced by FastQC (version 0.11.5)