FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-BulkA1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-BulkA1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17338457
Sequences flagged as poor quality0
Sequence length28
%GC17

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGCT18850.015.8721915
CGAGCTT24650.015.8412996
GAGCTTT103400.015.8186187
GAGGTTT165250.015.7755677
GGGCTTT156150.015.666467
GTGCTTT125050.015.639627
GTGATTT117050.015.5714557
GACGTTT90800.015.5182027
AGGTTTT553100.015.4026658
GGTGCTT39650.015.3967526
GAGATTT97200.015.3677977
CGGGCTT35450.015.3281626
CGTGCTT27250.015.2986146
GACCTTT57400.015.2730067
GGGATTT142650.015.221367
CGACCTT12650.015.2169256
GCGGTTT158950.015.1967217
CAGGTTT127150.015.1650447
GCGCTTT90750.015.1146447
AGCTTTT361700.015.1111878
Produced by FastQC (version 0.11.5)